Add a Virtual Assay

1. Add a new assay Visualization.

2. Import the python script heatmap.py into protocols.

3. Import the figure heatmap.svg resulting from the script into dataset.

   • Directoryassays

     • DirectoryVisualization

       • Directorydataset

         • heatmap.svg
       • Directoryprotocols

         • heatmap.py
       • isa.assay.xlsx
       • README.md
   • …

4. Add a new annotation table to Visualization and click Create basic table!.

5. This will create the following building blocks:

   • Input [Sample Name]: Use File Picker to reference the proteomics output file.
   • Protocol Uri: Use File Picker to reference python script.
   • Output [Sample Name]: Use File Picker to reference to heatmap result file.

6. Rightclick then Edit to update the input and output columns to be Input [Data] and Output [Data] respectively.

   Input [Data]	Protocol Uri	Output [Data]
   sugar_result.csv	heatmap.py	heatmap.svg