Basic Structure of a Protocol

What should a well-written SOP actually look like?

A protocol should follow a predictable structure. This improves readability and reviewability.

Example file:

• DNA_Extraction.md

Recommended Structure

Section titled Recommended Structure

# DNA Extraction – CTAB Method

## Purpose
Brief description of what the protocol achieves.

## Materials
- CTAB buffer
- Proteinase K
- Chloroform

## Equipment
- Centrifuge
- Heat block

## Procedure
1. Grind tissue.
2. Add buffer.
3. Incubate at 55°C.

## Expected Results
Describe quality criteria.

## Troubleshooting
Common problems and solutions.

Why Structure Matters

Section titled Why Structure Matters

Consistent structure:

• Makes review easier
• Reduces ambiguity
• Improves reproducibility
• Supports automated parsing later

Structure is not decoration — it is part of scientific rigor.