Mixed samples

How can I annotate the pooling or mixing of samples in annotation tables?

This example can be of relevance when you are carrying out labeling experiments or when you are spiking your samples with an internal standard for absolute quantification. The isa.assay.xlsx file below displays the best practice for annotating the mixing of experimental samples with a reference prior to LC/MS analysis (Component [Instrumentation]). By listing every data file twice, it becomes clear that the analyzed files originated from the combination of an experimental sample and a reference, e.g. spiking of S1 with the reference resulted in the output file name S1R.wiff.